Journal:

Article Title: Arylamine N -acetyltransferases: a new inhibitor of apoptosis in HepG2 cells *

doi: 10.1631/jzus.B0820090

Figure Lengend Snippet: Effect of 0.4 μg/ml camptothecin (CAM) on the morphology of HepG2 cells observed using confocal microscopy with acridine orange (AO)/ethidium bromide (EB) double staining. (a) Control; (b) 0.4 µg/ml CAM group

Article Snippet: Cell line Human hepatocarcinoma cell line HepG2 was originally purchased from American Type Culture Collection (ATCC), USA and was maintained by the Institute of Cancer Studies of Heilongjiang Cancer Hospital, China.

Techniques: Confocal Microscopy, Double Staining, Control

Journal:

Article Title: Arylamine N -acetyltransferases: a new inhibitor of apoptosis in HepG2 cells *

doi: 10.1631/jzus.B0820090

Figure Lengend Snippet: Effect of 0.4 μg/ml camptothecin (CAM) on the apoptosis rate of HepG2 cells observed using flow cytometry with PI single staining. (a) Control; (b) 0.4 µg/ml CAM group %G1: Percent of G1 phase; %S: Percent of S phase; %G2: Percent of G2 phase; %Tot: Apoptosis rate, i.e., percent of sub-G0 phase; Cell No.: Cell numbers determined

Article Snippet: Cell line Human hepatocarcinoma cell line HepG2 was originally purchased from American Type Culture Collection (ATCC), USA and was maintained by the Institute of Cancer Studies of Heilongjiang Cancer Hospital, China.

Techniques: Flow Cytometry, Staining, Control

Journal:

Article Title: Arylamine N -acetyltransferases: a new inhibitor of apoptosis in HepG2 cells *

doi: 10.1631/jzus.B0820090

Figure Lengend Snippet: The effect of CAM on NAT activity in HepG2 cells observed using HPLC. (a) 2-AAF standard; (b) Control; (c) 0.4 µg/ml CAM group

Article Snippet: Cell line Human hepatocarcinoma cell line HepG2 was originally purchased from American Type Culture Collection (ATCC), USA and was maintained by the Institute of Cancer Studies of Heilongjiang Cancer Hospital, China.

Techniques: Activity Assay, Control

Journal:

Article Title: Arylamine N -acetyltransferases: a new inhibitor of apoptosis in HepG2 cells *

doi: 10.1631/jzus.B0820090

Figure Lengend Snippet: Changes of NAT activity in apoptotic HepG2 cells induced by CAM

Article Snippet: Cell line Human hepatocarcinoma cell line HepG2 was originally purchased from American Type Culture Collection (ATCC), USA and was maintained by the Institute of Cancer Studies of Heilongjiang Cancer Hospital, China.

Techniques: Activity Assay

Journal:

Article Title: Arylamine N -acetyltransferases: a new inhibitor of apoptosis in HepG2 cells *

doi: 10.1631/jzus.B0820090

Figure Lengend Snippet: The effect of iodoacetamide on the induction of apoptosis in HepG2 cells by CAM observed using flow cytometry. (a) Control; (b) 0.08 μg/ml CAM, apoptosis rate: 10.4%; (c) 0.08 μg/ml CAM+100 μmol/L idoacetamide, apoptosis rate: 17.4%; (d) 0.08 μg/ml CAM+500 μmol/L iodoacetamide, apoptosis rate: 30.6%; (e) 500 μmol/L iodoacetamide %G1: Percent of G1 phase; %S: Percent of S phase; %G2: Percent of G2 phase; %Tot: Apoptosis rate, i.e., percent of sub-G0 phase; Cell No., Cell numbers determined

Article Snippet: Cell line Human hepatocarcinoma cell line HepG2 was originally purchased from American Type Culture Collection (ATCC), USA and was maintained by the Institute of Cancer Studies of Heilongjiang Cancer Hospital, China.

Techniques: Flow Cytometry, Control

Journal: PLoS ONE

Article Title: Studies on the non-invasive anticancer remedy of the triple combination of epigallocatechin gallate, pulsed electric field, and ultrasound

doi: 10.1371/journal.pone.0201920

Figure Lengend Snippet: The cell viability (24 h) was determined using MTT assay. (A) The HepG2 cells were triple treated with the EGCG (0 to 200 μM), 0.3 W/cm 2 US exposure (60 min), and consecutive 60 V/cm PEF for 24 h. The result revealed that the triple treatment could overcome the tolerance of cancer cells to EGCG and cause the death of HepG2 cells. (B) The triple treatment decreased the phosphorylation of Akt protein and increased the conversion of LC3-I to LC3-II in the HepG2 cells. The band intensities of LC3-II and LC3-I were quantified to calculate the LC3-II/LC3-I ratio. (C) The triple treatment triggered the activations of caspase-3 and PARP in the HepG2 cells. The cleaved PARP was quantified relative to the full-length PARP. β-actin was used as a loading control for each Western blot assay. (*** is used for P < 0.001).

Article Snippet: The human pancreatic cancer cell line PANC-1, the human hepatocellular carcinoma cell line HepG2, and the human embryonic kidney 293 (HEK293) were obtained from Bioresource Collection and Research Center (BCRC) (Hsinchu, Taiwan), and each STR-PCR profile for cell line authentication has been well recognized at BCRC.

Techniques: MTT Assay, Phospho-proteomics, Control, Western Blot

Journal: PLoS ONE

Article Title: Bi-Module Sensing Device to In Situ Quantitatively Detect Hydrogen Peroxide Released from Migrating Tumor Cells

doi: 10.1371/journal.pone.0127610

Figure Lengend Snippet: (A) in situ amperometric signal of melanoma A375 cell, larynx HEp-2 cell and liver cancer Hep G2 cell seeded in device containing conditional medium (B) histogram of maximum current signal during 12h of cells; (C) H&E staining of migrating A375, Hep G2 and HEp-2 cells; (D) histogram of increased migrating cell percentage using cells loaded in a device without serum in bottom chamber as a reference, * denotes p <0.05, n = 3.

Article Snippet: Human liver carcinoma cell line Hep G2 and human Larynx carcinoma cell line HEp-2, gifts from Dr. Yuan Li (Chongqing Medical University), were originally purchased from China Center for Type Culture Collection.

Techniques: In Situ, Staining